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3D super-resolution live-cell imaging with radial symmetry and Fourier light-field microscopy

https://doi.org/10.1364/BOE.471967

Han, Keyi ; Hua, Xuanwen ; Vasani, Vishwa ; Kim, Ge-Ah R. ; Liu, Wenhao ; Takayama, Shuichi ; Jia, Shu ( October 2022 , Biomedical Optics Express)

Live-cell imaging reveals the phenotypes and mechanisms of cellular function and their dysfunction that underscore cell physiology, development, and pathology. Here, we report a 3D super-resolution live-cell microscopy method by integrating radiality analysis and Fourier light-field microscopy (rad-FLFM). We demonstrated the method using various live-cell specimens, including actins in Hela cells, microtubules in mammary organoid cells, and peroxisomes in COS-7 cells. Compared with conventional wide-field microscopy,rad-FLFM realizes scanning-free, volumetric 3D live-cell imaging with sub-diffraction-limited resolution of ∼150 nm (x-y) and 300 nm (z), milliseconds volume acquisition time, six-fold extended depth of focus of ∼6 µm, and low photodamage. The method provides a promising avenue to explore spatiotemporal-challenging subcellular processes in a wide range of cell biological research.

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